Evaluation of Hepatitis C Virus Core Antigen Assays in Detecting Recombinant Viral Antigens of Various Genotypes

Hepatitis C Virus: Viral Quasispecies and Genotypes

Hepatitis C virus (HCV) mainly replicates in the cytoplasm, where it easily establishes persistent infection, resulting in chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Due to its high rate of mutation, HCV forms viral quasispecies, categorized based on the highly variable regions in the envelope protein and nonstructural 5A protein. HCV possesses seven major genotypes, amon...

the evaluation of hepatitis c virus core antigen in immunized balb/c mice

Distribution of Hepatitis C virus genotypes in Yazd, Central Province of Iran: increasing the mixed genotypes

Background and Aims: Different hepatitis C virus (HCV) genotypes have characteristic geographical distribution. Identification of HCV genotype is an important factor in the progression, clinical outcome and therapy of HCV infection. The aim of this study was to determine the prevalence of HCV genotypes among HCV-RNA positive patients in Yazd, Iran. Materials and Methods: In this cross-sectiona...

Expression of Hepatitis B Virus Core Antigen in Native and Fusion Forms in E. coli

DNA coding for the core antigen from hepatitis B Virus (HBcAg) was amplified, cloned and propagated in E. coli. The core protein was expressed in E. coli and the product was readily detected by Western blot. This protein can be used as a diagnostic material in serum screening tests. To increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...

CpG Motif as an Adjuvant in Immunization of a Recombinant Plasmid Encoding Hepatitis C Virus Core Protein